1064 nm laser induced thermal injure in mice skin with different laser duration
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摘要: 通过实验和理论分析的方法研究1064 nm激光不同辐照时间对小鼠皮肤的热损伤规律。利用皮肤镜图像和光学相干断层图像评估小鼠皮肤组织热损伤程度,利用Arrhenius热损伤方程计算热损伤参数,建立激光诱导皮肤组织热损伤模型,并与实验结果进行对比。结果表明,在靶功率密度为30 W/mm2的1064 nm激光辐照下,0~100 ms辐照时间内,小鼠皮肤组织损伤可恢复;150~280 ms辐照时间内,小鼠皮肤组织出现水肿现象和热凝固损伤;280~550 ms辐照时间内,小鼠皮肤表皮层出现汽化现象,损伤斑周围出现焦痂,真皮层发生变性;660 ms辐照时间以上,小鼠皮肤表皮层和真皮层出现汽化现象,伤口渗出组织液,皮下组织发生变性。理论分析与实验结果一致,建立的热损伤模型能够验证小鼠皮肤热损伤程度。Abstract: To explore laser induced thermal injure in skin tissue, 1064 nm laser induced thermal injure in mice skin with different laser duration was studied by experimental and theoretical analysis methods. Dermoscope images and optical coherence tomography images were used to assess the degree of thermal injure in biological skin tissue. Arrhenius thermal injure equation was used to calculate thermal damage parameters and establish a laser induced thermal injure model of skin tissue, then compared with experimental results. The results show that under the 1064 nm laser irradiation with 30 W/mm2 power density, there was reversible damage in the mouse skin tissue within 0 to 100 ms laser duration; the mice skin tissue appeared edema and thermal coagulation injure within 150 to 280 ms laser duration; during the laser duration of 280 to 550 ms, the epidermis of the mice skin was vaporized, eschars appeared around the damage spot, and the dermis was degenerated. Over 660 ms laser duration, the epidermis and dermis of the mice skin were vaporized, tissue fluid was exuded from the wound and the subcutaneous tissue was degenerated. The theoretical analysis is consistent with the experimental results. The established thermal injury model can verify the degree of thermal injury in mice skin.
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表 1 1064 nm激光诱导生物皮肤组织的热损伤分级
Table 1. Classification of 1064 nm laser induced thermal injure in biological skin tissue
laser duration/ms damage appearance 0~100 first-degree injure White coagulation spots appear on the skin epidermis, and the epidermis is flat 150~210 second-degree injure Blisters appear on the skin epidermis, with raised white coagulation spots 280~550 third-degree injure The epidermis of the skin is slightly vaporized, the dermis is degenerated, and shallow pit-like light red blood spots 660~$ \infty $ fourth-degree injure The skin epidermis and superficial dermis are vaporized, accompanied by exudation of tissue fluid, eschars appear on the edges of the wound, and deep pit-like crimson blood spots 表 2 热损伤程度和激光辐照时间关系表
Table 2. Relationship between laser duration and degree of thermal injure
laser duration/m Ω 60 3.51×10−10 100 3.86×10−19 150 4.39 210 208.73 280 19607.46 360 4.62×1013 550 6.52×1014 660 2.12×1015 780 5.59×1023 -
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