Experimental study on spores treatment by array plasma jet
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摘要: 研制了一套单极性微秒脉冲阵列式等离子体射流系统，该系统可在大气压下激发产生等离子体射流，实现大面积的灭菌处理。该系统可产生峰值电压20 kV、频率15 kHz的高压脉冲，激发产生的射流均匀稳定，覆盖面积达37.7 cm2，射流长度达6 cm，射流功率为40.05 W，处理5 min可使射流覆盖范围内的枯草芽孢杆菌的芽孢基本全部失去活性。考察了不同参数对灭菌效率的影响，结果表明，灭菌率与工作电压、脉冲频率、处理时间呈正相关，在氦气氛围下有较好的灭菌效果。SEM显示等离子体射流能够对枯草芽孢杆菌的芽孢外壳结构造成损坏，导致芽孢无法正常代谢，最终死亡。Abstract: In recent years, with the advancement in the field of biomedical applications, atmospheric pressure non-thermal plasma technology has shown many advantages in disinfection and sterilization. In terms of its application, the key lies in how to easily generate a large-area, uniform and stable plasma. In this work, a set of unipolar microsecond pulse array plasma jet system is developed, which can be excited to generate plasma jet under atmospheric pressure and realize large-area sterilization treatment. The system generates high-voltage pulses with a peak voltage of 20 kV and a frequency of 15 kHz. The jet is uniform and stable, covering an area of 37.7 cm2, the jet length is stable at 6 cm and the jet power is 40.05 W. Treating for 5 min can basically inactivate all Bacillus subtilis spores in the area covered by the jet. The effects of different parameters on the sterilization efficiency are investigated, and the results show that the sterilization rate is positively correlated with the working voltage, pulse frequency and treatment time. The sterilization effect is better in helium atmosphere. The SEM images show that the plasma jet damaged the shell structure of Bacillus subtilis spores, hence the spores failed to metabolize normally and eventually died.
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图 6 脉冲等离子体射流的电压及电流波形(a)和单次脉冲能量(b)
Figure 6. Voltage and current waveform (a) and single pulse energy (b) of pulsed plasma jet
图 7 不同气体成分下等离子体射流的发射光谱
Figure 7. Emission spectra of plasma jets with different gas compositions
图 10 不同脉冲频率下射流的灭菌效果
Figure 10. Sterilizing effect of jet at different pulse frequencies
图 11 不同处理时间下射流的灭菌效果
Figure 11. Sterilizing effect of jet under different treatment time
图 12 不同气体氛围下射流的灭菌效果
Figure 12. Sterilization effect of jet under different gas atmosphere
图 13 不同气体氛围下射流的灭菌实验数据
Figure 13. Sterilization data of jet under different gas atmosphere
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