Abstract: A new phenomenon about crosstalk effect of CCD was discovered in the experiment of laser irradiating the interline transfer CCD camera. It is that a gap, adjoining the upper side of the main spot, appears on the crosstalk line and becomes smaller when laser becomes more intense. Through the analysis on the structure and process of interline transfer CCD image sensor, a new crosstalk mechanism is given to explain the phenomenon. In the process of vertical transfer action, the overflowing of signal charges in integral well to vertical CCD register makes the crosstalk line. When readout transfer action empties the integral well, the integral well needs time to be refilled, during which no signal charge overflows to vertical CCD register. While the main signal charge package that makes the ma