Abstract: The design method of hardware-in-the-loop simulation platform for measurement and control system is introduced. The system hardware is implemented by using computer and PCI interface card, and the electric characteristics of control interface are simulated veritably. The control function of correlative sub-system is simulated according to veritable communication principles including principles of shared-memory communication and CAN communication, etc. The simulation platform is successfully applied to measurement and control system, and the quality of the system is improved.