Abstract: The inconsistency of multi-channels digital to analog conversion circuit output seriously affects the simulation quality of the infrared detector simulator. In order to solve this problem, a novel method called two-point inconsistency calibration model (TICM) is proposed by introducing a two-point non-uniformity correction algorithm into the inconsistency problem. By analyzing the sample data of the infrared detector simulator, correction factors are obtained, and the TICM is implemented on the core processor FPGA (field-programmable gate array). The images before and after inconsistency correction are obtained by simulating the output signal of 320256 mid-wave infrared cooling detector. The result shows that the maximum fluctuation of the column average gray level is up to 15 initially, but after the inconsistency correction, it decreases to 5. There is about a 66.7% increase in output performance by using TICM. Thus, the established inconsistency calibration model is a good solution to the inconsistency problem of multi-channels digital to analog conversion circuit output and provides a foundation for quantitative simulation of the infrared detector simulator.