Analysis of inactivation saccharomyces cerevisiae by 13 μs, 200 ns and 2 ns pulsed electric fields

The effect of the pulse width change is studied on the membrane voltage and energy of cell membranes and organelles membrane under the high voltage pulse electric field. The sterilization effect of saccharomyces cerevisiae (CICC31180) changes with the quantity of the 13 s, 200 ns or 2 ns pulses. The results showed that with the increase of the quantity of pulses, the sterilization effect was enhanced. The yeast concentration decreased by 4.3 orders of magnitude after 50, 13 s width, 14 kV pulses treatment while by 2.8 orders of magnitude after 500, 13 ns width 200 kV pulses treatment, by 2.6 orders of magnitude after 500, 13 ns width 200 kV pulses treatment. The survival rate logarithmic relation with the pulse number was not linear. The survival rate logarithmic decreased slowly when the pulse number increased to a higher value. Under the same survival rate logarithmic decrease, the loss of energy in coaxial electrode was lower in 2 ns pulses than 13 s or 200 ns pulses.