Surface-enhanced Raman effect of new coronavirus S protein in gold nanoparticles

Le Wei; Huang Jinglin; Yang Qiang; Qi Daojian; Ni Shuang; Wei Lai; Fan Quanping; Yang Yue; Wen Jiaxing; Mo Wenbo; Chen Guo; Zhao Songnan; He Zhibing; Li Bo; Du Kai

doi:10.11884/HPLPB202133.210466

Volume 33 Issue 11

Nov. 2021

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Le Wei, Huang Jinglin, Yang Qiang, et al. Surface-enhanced Raman effect of new coronavirus S protein in gold nanoparticles[J]. High Power Laser and Particle Beams, 2021, 33: 119001. doi: 10.11884/HPLPB202133.210466

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Le Wei, Huang Jinglin, Yang Qiang, et al. Surface-enhanced Raman effect of new coronavirus S protein in gold nanoparticles[J]. High Power Laser and Particle Beams, 2021, 33: 119001. doi: 10.11884/HPLPB202133.210466

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Surface-enhanced Raman effect of new coronavirus S protein in gold nanoparticles

doi: 10.11884/HPLPB202133.210466

Le Wei^1
,,
Huang Jinglin¹,
Yang Qiang²,
Qi Daojian¹,
Ni Shuang¹,
Wei Lai^{1, 3},
Fan Quanping^{1, 3},
Yang Yue^{1, 3},
Wen Jiaxing³,
Mo Wenbo³,
Chen Guo¹,
Zhao Songnan¹,
He Zhibing¹,
Li Bo¹,
Du Kai^{1
,
,}

1.
Laser Fusion Research Center, CAEP, Mianyang 621900, China
2.
China Academy of Engineering Physics, Mianyang 621900, China
3.
Science and Technology on Plasma Physics Laboratory, Mianyang 621900, China

Received Date: 2021-10-20
Rev Recd Date: 2021-11-10

Available Online: 2021-11-15

Publish Date: 2021-11-15

Abstract

Abstract

Surface-enhanced Raman spectroscopy (SERS) technology has been widely used in viral molecular detection due to its high sensitivity, simple operation and rapid detection. The research of virus detection by Raman technology at home and abroad mainly focuses on the detection of the SERS spectrum of viral nucleic acids and various bases that make up the nucleic acids, and detection of viral proteins is rare. In this paper, the S protein of the new coronavirus (SARS-CoV-2) is used as the detection object, and with the label-free SERS detection method, the ordinary Raman spectra of solid and saturated liquid S protein of the SARS-CoV-2 and the SERS spectra of the low-concentration S protein of SARS-CoV-2 on the substrate of gold nanoparticles with a size of 40 nm are compared. The results show that it is completely feasible to use SERS technology to detect the S protein of SARS-CoV-2 on the substrate of 40 nm gold nanoparticles. The carboxyl groups in the S protein molecule of SARS-CoV-2 and gold nanoparticles are molecularly enhanced, and the amino groups and gold nanoparticles are electromagnetically enhanced, so that the Raman effect of the S protein of the SARS-CoV-2 is enhanced and the peak position is moved to a certain extent. The experiments obtained relatively good SERS spectra of the low-concentration S protein of SARS-CoV-2, which provides a method for the establishment of a sensitive, specific and rapid detection technology for the S protein of the SARS-CoV-2.
- surface-enhanced Raman spectroscopy technology,
- severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2),
- gold nanoparticles,
- protein,
- interaction

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References(19)

References

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