Before making wind measurements, it is vital to calibrate the new developed Doppler wind lidar system, which can demonstrate and improve the validity of the lidar system. Based on the principle of wind measurement, a new method for the calibration of Rayleigh Doppler wind lidar taking advantage of the relationship between Rayleigh scattering spectrum and Mie scattering spectrum is proposed. This method uses a moving hard target to calibrate the Rayleigh Doppler wind lidar. The experimental system is designed for Rayleigh Doppler lidar calibration and the detailed processes are given as well.