A high sensitive radiochromic film has been developed by using poly (vinyl butyral) as the base and using acetylene-type compounds as the organic dye material, and was tested with the J-2.5 proton electrostatic accelerator, with proton energy of 2.0 MeV, irradiation fluence of 1.0×1010 to 1.0×1012 cm2. The tests of radiation effects show that, the color of the radiochromic film changes from pink to blue, and the optical density decreases along with the increase of the focus spot distance. Absorbance spectrum analysis shows that, the peak in the visual region locates around 660 nm. There is a linear relationship between absorbency and proton-flux for irradiation at the same proton energy. The response of the film can be measured immediately after irradiati